Superior sensitivity by seamlessly interfacing to the Agilent 6100

The applicability of Supercritical Fluid Chromatography (SFC) can be substantially enhanced by coupling SFC to Mass Spectrometric detection (MSD). In this Technical Overview, two instrument configurations are described that can be used to connect the Agilent 1260 Infinity Analytical SFC to a mass spectrometer either using an atmospheric pressure chemical ionization (APCI) or an electrospray (ESI) source. Tests were performed using an Agilent 6130 Single Quadrupole LC/MS, however all other Agilent Single Quadrupole LC/MS systems can also be used. In the proposed configurations, no effluent splitting is used. Good repeatability (retention time RSD < 0.3% and peak area RSD < 10%) and sensitivity (10 × more sensitive than UV detection) are obtained while minimal peak dispersion is observed compared to UV/DAD detection. Authors Melissa Dunkle, Gerd Vanhoenacker, Frank David, and Pat Sandra Research Institute for Chromatography President Kennedypark 26 8500 Kortrijk, Belgium Martin Vollmer Agilent Technologies, Inc. Waldbronn, Germany Technical Overview min 2 4 6 8 10 12 14 16